Observations and analysis Week 4.
All columns appear to show intense development of both purple non-S bacteria and purple S bacteria in anaerobic water. It is hard to compare each occluded column with the control one without disturbing its environment; therefore the final conclusion will be made during the last observation, when the cardboard is taken off the bottles. Nevertheless, during the forth week besides purple bacteria intense development in each bottle, there appears to be green S bacteria underneath. There is no evidence of bubbles (Hydrogen Sulfide gas) anymore, as it has all been consumed by non-aerobic bacteria breaking organic matter down. No foul odor either. The most intense odor was during the first two-three weeks.
1. The top occluded bottle: this bottle shows better development of green S bacteria underneath the purple S bacteria than the control one. Furthermore, photosynthetic aerobic bacteria is supposed to show less growth according to our hypothesis, nonetheless it develops very well on the water surface as well as in the top layer. Less intense purple color in the middle of the bottle.
2. The middle occluded bottle: there is the same intense purple color, as in the control one. No access to see the S bacteria underneath. The top layer is well developed.
3. The bottom occluded bottle: the same development of photosynthetic bacteria on top as well as purple non-S, S bacteria, and green S bacteria. There seems to be no difference between the control bottle and this bottle.
Things to consider: our columns are located right on top of the radiator. Since the weather is getting colder, the radiator started working and heats the columns and the room. Does increase in temperature stimulates the bacterial growth?
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